Molecular analysis and genome discovery [[electronic resource] /] / edited by Ralph Rapley and Stuart Harbron

• Record Nr.: UNINA9910139595203321
• Pubbl/distr/stampa: Chichester, West Sussex ; ; Hoboken, NJ, : John Wiley & Sons, 2011
• ISBN: 1-283-28453-7; 9786613284532; 1-119-97743-6; 1-119-97744-4
• Edizione: [2nd ed.]
• Descrizione fisica: 1 online resource (317 p.)
• Altri autori (Persone): RapleyRalph; HarbronStuart
• Disciplina: 615/.7
• Soggetti: Molecular diagnosis; Genomics; Proteomics; Pharmacogenomics; Polymerase chain reaction; DNA microarrays
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: Molecular Analysis and Genome Discovery; Contents; Preface; Contributors; 1 Overview of Genotyping; Introduction; Methods for interrogating SNPs; Commercial platforms for SNP genotyping; Practical recommendations; SNP databases; Methylation analysis; Copy number variation analysis; Second generation sequencing technologies; Conclusions; References; 2 DNA Chip Analysis in Genome Discovery; Introduction; Interrogating a genome; Cross-species hybridization; Comparative genomic hybridization and microarray-based genotyping; Barcodes, DNA microarrays and organism identification; Concluding remarks; References3 qPCR, Theory, Reliability and Use in Molecular Analysis; Sample preparation; RNA quality; Reagents; Assay design; Transparency of published data; Further considerations; Conclusion; References; 4 DNA Analysis in Droplet-Based Microfluidic Devices; Introduction; ; Continuous-flow microPCR chips; PCR inhibition and carryover contamination; PCR in droplets; Conclusions; References; 5 High-Resolution Melt Profiling; Introduction; Basic concepts of melt profiling; HRMP and polymerase chain reaction; DNA specimens and HRMP; Determining a temperature window for melting; Dyes and platforms for melt profilingScanning PCR products for sequence variation; Genotyping with high-resolution melt profiling; Other applications of HRMP; Final notes; References; 6 Massively Parallel Sequencing; Sanger sequencing; Massively parallel sequencing; Commercially available massively parallel sequencers; Future technologies; Paired-end or mate-paired reads; Target-enrichment strategies for MPS; Applications of MPS; Summary; References; 7 Aptamers for Analysis: Nucleic Acids Ligands in the Post-Genomic Era; Introduction; SELEX; Aptamers in analysis; Imaging with aptamers; Conclusions, outlooks and perspectivesReferences; 8 Use of Nanotechnology for Enhancing of Cancer Biomarker Discovery and Analysis: A Molecular Approach; Introduction; Proteomics and nanotechnology; Nanoscale multicomponent separation; Nanoscale protein detection strategies; Surface-enhanced Raman scattering (SERS); References; 9 Chip-Based Proteomics; Introduction; Lab-on-a-chip; Arrays; Chip-based mass spectrometry; Surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) chip instruments; Microfluidics; Conclusion; References; 10 Antibody Microarrays in Proteome Profiling; IntroductionTechnical aspects; Antibody array applications; Summary; Acknowledgements; References; 11 Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics; Introduction; Principles of microbead-based multiplexing; Experimental aspects of the multiplex microbead assay; Multiplex microbead assay design and comparison with other methods; Applications of the multiplex microbead assay system for biomedical research and clinical studies; Selected investigational fields for multiplex analysis and examples of applications; Challenges and current limitations
• Sommario/riassunto: Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and developed. Each chapter offers an up-to-date analysis of the subject encompassing the very latest technology platforms and is an essential reference for researchers in the field looking for an u