Platelet proteomics : principles, analysis, and applications / / edited by Angel Garcia, Yotis Senis

• Record Nr.: UNINA9910139450803321
• Pubbl/distr/stampa: Hoboken, NJ, : John Wiley & Sons, c2011
• ISBN: 1-118-00207-5; 1-283-08201-2; 9786613082015; 0-470-94029-8; 0-470-94028-X
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (425 p.)
• Collana: Wiley-Interscience series on mass spectrometry
• Altri autori (Persone): GarciaAngel <1968->; SenisYotis A. <1970->
• Disciplina: 612.1/17
• Soggetti: Blood platelets; Proteomics
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographical references and index.
• Nota di contenuto: PLATELET PROTEOMICS; CONTENTS; Contributors; Foreword; Preface; Acronyms; PART I GENERAL OVERVIEW: PLATELETS, SAMPLE PREPARATION, AND MASS SPECTROMETRY-BASED PROTEOMICS; 1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis; 2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research; 3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research; PART II ANALYSIS OF THE PLATELET PROTEOME: GLOBAL APPROACHES AND SUBPROTEOMES; 4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies5 The Platelet Membrane Proteome; 6 Proteomics of Platelet Granules, Organelles, and Releasate; 7 The Platelet Microparticle Proteome; 8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome; PART III INTEGRATED "OMICS" AND APPLICATION TO DISEASE; 9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome; 10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders11 Platelet Functional Genomics; 12 Systems Biology to Study Platelet-Related Bleeding Disorders; 13 Platelet Proteomics in Transfusion Medicine; 14 Cardiovascular Proteomics; Index
• Sommario/riassunto: The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separat