1.

Record Nr.

UNINA9910132350203321

Autore

Barbosa Pedro <1944->

Titolo

Insect histology : practical laboratory techniques / / Pedro Barbosa, Deborah L. Berry, Christina S. Kary

Pubbl/distr/stampa

West Sussex, England : , : John Wiley & Sons, , 2015

©2015

ISBN

1-4443-3695-9

1-118-87611-3

1-118-87500-1

1-118-87571-0

Edizione

[Second edition.]

Descrizione fisica

1 online resource (397 p.)

Disciplina

595.7

Soggetti

Entomology

Histology

Lingua di pubblicazione

Inglese

Formato

Materiale a stampa

Livello bibliografico

Monografia

Note generali

Description based upon print version of record.

Nota di bibliografia

Includes bibliographical references and index.

Nota di contenuto

Problems of sclerotized chitin : softening insect cuticle -- Fixation -- Dehydration, clearing, and embedding -- Staining -- Immunohistochemical staining -- Use of genetic markers in insect histology -- Fluorescence -- Mounting -- Preparation of whole mounts -- Whole mount staining -- Preparation of insect tissues -- Preparation of genitalia, mouthparts, and other body parts -- Preparation of chromosomes -- Appendix : dissecting fluids and insect saline solutions.

Sommario/riassunto

This title is a much needed update of Barbosa''s self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of ''traditional'' and ''modern'' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. Insect Histology is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.