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Record Nr. |
UNINA9910132186803321 |
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Titolo |
Biofouling methods / / edited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams |
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Pubbl/distr/stampa |
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West Sussex, England : , : John Wiley & Sons, Ltd., , 2014 |
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©2014 |
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ISBN |
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1-118-33611-9 |
1-118-33614-3 |
1-118-33613-5 |
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Edizione |
[First edition.] |
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Descrizione fisica |
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1 online resource (411 p.) |
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Disciplina |
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Soggetti |
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Fouling |
Fouling organisms |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Nota di bibliografia |
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Includes bibliographical references and index. |
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Nota di contenuto |
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Biofouling Methods; Copyright; Contents; List of contributors; Introduction; Guide to methods; Part I Methods for Microfouling ; Chapter 1 Microscopy of biofilms; Section 1: Traditional light and epifluorescent microscopy; 1.1 Introduction; 1.2 Determination of bacterial abundance; 1.3 Catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH); 1.4 Suggestions, with examples, for data analysis and presentation; Acknowledgements; References; Chapter 2 Traditional and bulk methods for biofilms; Section 1: Traditional microbiological methods; 2.1 Introduction |
2.2 Enrichment culture, isolation of microbes 2.3 Counting methods; 2.4 Troubleshooting hints and tips; References; Section 2 Bulk methods; 2.5 Introduction; 2.6 Measurement of biofilm thickness; 2.7 Biofilm dry weight determination; 2.8 Biofilm ATP content; 2.9 Troubleshooting hints and tips; Acknowledgements; References; Chapter 3 Biocide testing against microbes; Section 1: Testing biocides in solution: flow cytometry for planktonic stages; 3.1 Introduction; 3.2 Method introductions; 3.3 Pros and cons; 3.4 Materials and equipment; 3.5 Methods; 3.6 Troubleshooting hints and tips |
3.7 Suggestions References; Section 2 Biocide testing using single and |
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multispecies biofilms; 3.8 Introduction; 3.9 Questions to answer when applying biocides; 3.10 Laboratory methods for testing biocide effect; 3.11 Field methods for testing biocide effect; 3.12 Troubleshooting hints and tips; Acknowledgements; References; Chapter 4 Molecular methods for biofilms; Section 1: Isolation of nucleic acids; 4.1 Introduction; 4.2 Materials; 4.3 Isolation of DNA from a biofilm; 4.4 Troubleshooting hints and tips; References; Section 2 PCR and DNA sequencing |
4.5 PCR and DNA sequencing: General introduction 4.6 PCR; 4.7 Microbial marker genes - 16S; 4.8 DNA sequencing; 4.9 454 16S amplicon pyrotag sequencing; 4.10 Protocol 1: DNA extraction using the Qiagen DNeasy Plant Mini Kit; 4.11 Protocol 2: Full-length 16S PCR using the Qiagen Multiplex Kit; 4.12 Protocol 3: Analysis of full-length 16S genes; 4.13 Protocol 4: 16S amplicon PCR for 454 sequencing using the Qiagen Multiplex Kit; 4.14 Protocol 5: Trimming and filtering of 454 16S pyrotag sequencing; 4.15 Protocol 6: Taxon-based analyses; 4.16 Protocol 7: Phylogeny-based analyses; References |
Section 3 Community comparison by genetic fingerprinting techniques 4.17 Introduction; 4.18 History and principles of the methods; 4.19 Advantages and limitations of fingerprinting techniques; 4.20 Materials and equipment; 4.21 Suggestions for data analysis and presentation; 4.22 Troubleshooting hints and tips; Acknowledgements; References; Section 4 Metagenomics; 4.23 Introduction and brief summary of methods; 4.24 Overview of metagenomics methods; 4.25 Method introduction; 4.26 Overview of DNA handling for BAC library construction; 4.27 BAC and Fosmid library construction |
4.28 Library handling, archiving, and databasing |
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Sommario/riassunto |
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Biofouling Methods provides a "cook book" for both established workers and those new to the field. The methods included in this important new book range from tried and tested techniques to those at the cutting edge, encompassing the full diversity of this multidisciplinary field. The book covers methods for microbial and macrofouling, coatings and biocides, and ranges from methods for fundamental studies to methods relevant for industrial applications. There is an emphasis on answering questions and each chapter provides technical methods and problem-solving hints and tips. Bringing togethe |
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