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Record Nr. |
UNINA9910967020903321 |
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Titolo |
Developing minority language resources : the case of Spanish in California / / Guadalupe Valde s ... [et al.] |
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Pubbl/distr/stampa |
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Clevedon, : Multilingual Matters, 2006 |
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ISBN |
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9786610507788 |
9781280507786 |
1280507780 |
9781853598999 |
1853598992 |
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Edizione |
[1st ed.] |
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Descrizione fisica |
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1 online resource (xxi, 317 pages) |
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Collana |
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Bilingual Education & Bilingualism |
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Altri autori (Persone) |
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Disciplina |
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Soggetti |
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Spanish language - Study and teaching (Secondary) - California |
Spanish language - Study and teaching (Higher) - California |
Hispanic Americans - Education - California |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Description based upon print version of record. |
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Nota di bibliografia |
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Includes bibliographical references. |
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Nota di contenuto |
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Front matter -- Contents -- Acknowledgments -- Introduction -- Chapter 1. Acquisition, Maintenance, and Recovery of Heritage Languages -- Chapter 2. Three Hundred–Plus Years of Heritage Language Education in the United States -- Chapter 3. The Spanish Language in California -- Chapter 4. The Use of Spanish by Latino Professionals in California -- Chapter 5. The Foreign Language Teaching Profession and the Challenges of Developing Language Resources -- Chapter 6. Secondary Spanish Heritage Programs in California -- Chapter 7. Postsecondary Spanish Heritage Programs in California -- Chapter 8. The Teaching of Heritage Languages -- Chapter 9. Imagining Linguistic Pluralism in the United States -- Methodological Appendix |
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Sommario/riassunto |
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This book documents ongoing language shift to English among Latino professionals in California 67% of which studied Spanish formally in high school and 54% of which studied Spanish in college. Taking into account the recommendations about the teaching of Spanish as a heritage language made by these professionals, the book then |
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describes current instructional practices used in the teaching of Spanish as an academic subject at the high school and university levels to “heritage” language students who, although educated entirely in English, acquired Spanish at home as their first language. The suggestions made by the Professionals concentrated almost exclusively on Spanish language maintenance (e.g., making cultural/historical connections; showing relevance and significance of language to students’ lives, teaching other subjects in Spanish, teaching legal, medical, business terms in Spanish). The study of goals currently guiding instruction for heritage speakers of Spanish at both the high school and the college levels, on the other hand, raise questions about the potential contribution of educational institutions to the maintenance and retention of Spanish among the current Spanish-speaking population of California. |
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2. |
Record Nr. |
UNINA9910131313103321 |
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Autore |
Till Bradley J |
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Titolo |
Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger |
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Pubbl/distr/stampa |
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2015 |
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Cham : , : Springer International Publishing : , : Imprint : Springer, , 2015 |
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ISBN |
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Edizione |
[1st ed. 2015.] |
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Descrizione fisica |
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1 online resource (X, 35 p. 9 illus., 3 illus. in color.) |
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Classificazione |
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SCI007000SCI008000SCI011000 |
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Disciplina |
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Soggetti |
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Plant biotechnology |
Biology - Technique |
Biomaterials |
Nucleic acids |
Plant Biotechnology |
Biological Techniques |
Nucleic Acid |
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Lingua di pubblicazione |
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Formato |
Materiale a stampa |
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Livello bibliografico |
Monografia |
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Note generali |
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Bibliographic Level Mode of Issuance: Monograph |
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Nota di contenuto |
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Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . |
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Sommario/riassunto |
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This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. |
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