Advanced optical flow cytometry [[electronic resource] ] : methods and disease diagnoses / / edited by Valery V. Tuchin

• Record Nr.: UNINA9910131028303321
• Pubbl/distr/stampa: Weinheim, : Wiley-VCH, c2011
• ISBN: 3-527-63429-0; 3-527-63430-4; 3-527-63428-2
• Descrizione fisica: 1 online resource (741 p.)
• Altri autori (Persone): TuchinV. V (Valerii Viktorovich)
• Disciplina: 616.07582
• Soggetti: Flow cytometry - Diagnostic use; Cytometry; Electronic books.
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Description based upon print version of record.
• Nota di bibliografia: Includes bibliographic references and index.
• Nota di contenuto: Advanced Optical Flow Cytometry; Contents; Preface; List of Contributors; 1 Perspectives in Cytometry; 1.1 Background; 1.2 Basics of Cytometry; 1.2.1 Flow Cytometry; 1.2.2 Slide-Based Cytometry; 1.3 Cytomics; 1.4 Cytometry - State of the Art; 1.4.1 Multiparametric Analyses; 1.5 Perspectives; 1.5.1 New Technologies and Methods; 1.5.1.1 Sequential Analyses; 1.5.1.2 Spectral Analyses; 1.5.1.3 Fluorescence Modifications for Analyses; 1.5.1.4 Label-Free Analyses; 1.5.2 Automation; 1.5.3 Cytometry - the Other Side; 1.6 Conclusion; References; 2 Novel Concepts and Requirements in Cytometry; 2.1 Introduction2.2 Fluorescence Microscopy; 2.2.1 Light Dose; 2.2.2 Cell Systems; 2.2.3 Methods; 2.3 Fluorescence Reader Systems; 2.3.1 Cell-Based Fluorescence Screening; 2.3.2 TIR Fluorescence Reader; 2.4 Microfluidics Based on Optical Tweezers; 2.5 Conclusion; Acknowledgment; References; 3 Optical Imaging of Cells with Gold Nanoparticle Clusters as Light Scattering Contrast Agents: A Finite-Difference Time-Domain Approach to the Modeling of Flow Cytometry Configurations; 3.1 Introduction; 3.2 Fundamentals of the FDTD Method; 3.2.1 The Basic FDTD Numerical Scheme; 3.2.2 Input Wave Excitation3.2.3 Uniaxial Perfectly Matched Layer ; Absorbing Boundary Conditions; 3.2.4 FDTD Formulation of the Light Scattering Properties from Single Cells; 3.2.5 FDTD Formulation of Optical Phase Contrast Microscopic (OPCM) Imaging; 3.3 FDTD Simulation Results of Light Scattering Patterns from Single Cells; 3.3.1 Effect of Extracellular Medium Absorption on the Light Scattering Patterns; 3.4 FDTD OPCM Nanobioimaging Simulation Results; 3.4.1 Cell Structure; 3.4.2 Optical Clearing Effect; 3.4.3 The Cell Imaging Effect of Gold Nanoparticles; 3.4.3.1 A Cell with a Cluster of Gold Nanoparticles Located in the Cytoplasm3.4.3.2 A Cell with a Cluster of Gold Nanoparticles Randomly Distributed on the Surface of its Nucleus; 3.5 Conclusion; Acknowledgment; References; 4 Optics of White Blood Cells: Optical Models, Simulations, and Experiments; 4.1 Introduction; 4.1.1 White Blood Cells; 4.1.2 Particle Identification and Characterization; 4.1.3 Experimental Techniques; 4.2 Optical Models of White Blood Cells; 4.2.1 Confocal Imaging of White Blood Cells; 4.2.2 Optical Models of Mononuclear Cells; 4.2.3 Optical Models of Granular Cells; 4.2.4 Refractive Indices of White Blood Cells and their Organelles4.3 Direct and Inverse Light-Scattering Problems for White Blood Cells; 4.3.1 Simulation of Light Scattering by Mononuclear Cells; 4.3.2 Simulation of Light Scattering by Granular Cells; 4.3.2.1 Granulocyte Model Without Nucleus; 4.3.2.2 Approximate Theories; 4.3.2.3 Neutrophil Model with Nucleus; 4.3.3 Inverse Light-Scattering Problem for Mononuclear Cells; 4.3.3.1 Global Optimization; 4.3.3.2 Errors of Parameter Estimates; 4.3.3.3 Theoretical Tests Based on More Complicated Model; 4.3.3.4 Sample Characterization; 4.4 Experimental Measurement of Light Scattering by White Blood Cells
• Sommario/riassunto: A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman,