Summa totius Theologiæ S. Thomæ Aquinatis, Doctoris Angelici Ordinis Prædicatorum. Primæ [-tertiae] partis volumen primum [-quartum]

• Record Nr.: UNINA990010081270403321
• Autore: Tommaso d'Aquino, santo <1225?-1274>
• Pubbl/distr/stampa: Lugduni : sumptibus Anisson & Posuel, 1701-1702
• Descrizione fisica: 20 v. ; 12°
• Locazione: FGBC
• Collocazione: III N 105 (1.1); III N 105 (1.2); III N 105 (1.3); III N 105 (1.4); III N 105 (2.1.1); III N 105 (2.1.2); III N 105 (2.1.3); III N 105 (2.1.4); III N 105 (2.2.1); III N 105 (2.2.2); III N 105 (2.2.3); III N 105 (2.2.4); III N 105 (2.2.5); III N 105 (3.1); III N 105 (3.2); III N 105 (3.3); III N 105 (4.1); III N 105 (4.2); III N 105 (4.3); III N 105 (20)
• Lingua di pubblicazione: Latino
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Nota di contenuto: 1.1.-1.4.: Primæ partis volumen primum [-quartum]. - 1701 2.1.1.-2.1.4.: Primæ secundæ partis volumen primum [-quartum] . - 1701 ; 2.2.1.-2.2.5.: Secundæ secundæ partis volumen primum [-quintum]. - 1701 3.1.-3.3.: Tertiæ partis volumen primum [-tertium]. - 1702 4.1.-4.2.: Supplementi tertiæ partis Summæ Theologicæ S. Thomæ Aquinatis, ordinis Prædicatorum volumen primum [-tertium]. - 1702 Indices omnes in D. Thomæ Summam Theologicam hactenus impressi, nunc emendati, & in optimum ordinem ad invicem redacti. - 1702

Mutation detection : a practical approach / / edited by R.G.H. Cotton, E. Edkins and S. Forrest

• Record Nr.: UNINA9910955394303321
• Pubbl/distr/stampa: Oxford : , : IRL Press at Oxford University Press, , 2023
• ISBN: 1-383-04935-1; 0-19-156569-5; 1-280-37548-5; 9786610375486; 0-585-48413-9
• Edizione: [1st ed.]
• Descrizione fisica: 1 online resource (263 p.)
• Collana: Practical approach series; Oxford scholarship online; Practical approach series ; ; 188
• Disciplina: 576.5/49
• Soggetti: Mutation (Biology); Molecular genetics; Chromosome abnormalities
• Lingua di pubblicazione: Inglese
• Formato: Materiale a stampa
• Livello bibliografico: Monografia
• Note generali: Previously issued in print: 1998.
• Nota di bibliografia: Includes bibliographical references.
• Nota di contenuto: Cover; Contents; List of Contributors; Abbreviations; Introduction; References; 1. Single-strand conformation polymorphism analysis; 1. Introduction; 2. PCR-SSCP using polyacrylamide slab gel; PCR Optimization and primer design; Pre-amplification and isolation by agarose gel electrophoresis; PCR using [[Sup(32)]P]deoxynucleotide triphosphate; Removal of 3' appendage; SSCP gel electrophoresis; Interpretation of autoradiogram; Re-amplification and direct sequencing; Gel matrices other than polyacrylamide; Restriction ; endonuclease fingerprinting and dideoxy fingerprinting; 3. Fluorescent SSCP in an automated DNA sequencerPrimer design in post-PCR fluorescent labelling; Fluorescent labelling by 3' exchange reaction; SSCP in capillary electrophoresis (CE-SSCP); Data processing; Acknowledgements; References; 2. Single-stranded conformation polymorphism and heteroduplex analysis; 1. Introduction; 2. Optimization of the PCR reaction; 3. SSCP sample prepration; 4. Optimization of SSCP/HA detection; 5. Multiplexing; 6. Interpretation of results; 7. Applications; 8. Other methods; References; 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis1. Introduction; The scope of DGGE, its distinctive capabilities, and the nature of results; 2. Background; 3. Basic principle, the physical properties of DNA; 4. Overview of the procedures in searching for mutants; Defining segments for scrutiny; Sample preparation; Gradient and velocity separations; Features of the gel patterns; Discrimination of zygozygosity; Comments; 5. Use of the psoralen cross-link as a clamp; The psoralen protocol; 6. Computational tools; What is a meltmap?; Meltmap protocol; Predicting electrophoretic separationsComputer operations for MUTRAV; 7. Other members of the DGGE family; Gel separations in a uniform, partially denaturing environment; Capillary electrophoresis; The thermal gradient; The temperature ramp; 2D length and gradient separations; 8. End notes; Acknowledgments; References; 4. Cleavage using RNase to detect mutations; 1. Introduction; 2. RNase protection assay for mutation detection; Evaluation of the sensitivity; Source material; PCR for RNase protection assay; RNA probe preparation; RNase protection; Detection of digested probe; Mutation detection by sequencing of the PCR productsOther modified methodologies for mutation detection; Acknowledgements; References; 5. Cleavage of mismatched bases using chemical reagents; 1. Introduction; 2. Basic procedures; Comments on the basic procedures; 3. Ultra fast chemical mismatch detection; Labelling; Solid phase; Comments; References; 6. Mutation detection using T4 endonuclease VII; 1. Introduction; 2. The biology of Endo VII; The role of Endo VII in vivo; Characterization of Endo VII; Action of Endo VII on heteroduplex DNA; 3. Use of Endo VII for mutation detection; Enzyme mismatch cleavage
• Sommario/riassunto: This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.