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Chiral Separation Techniques; Contents; Preface; List of Contributors; 1 Method Development and Optimization of Enantioseparations Using Macrocyclic Glycopeptide Chiral Stationary Phases; 1.1 Introduction; 1.2 Structural Characteristics of Macrocyclic Glycopeptide CSPs; 1.2.1 Chiral Recognition Mechanisms; 1.2.2 Multi-modal Chiral Stationary Phases; 1.3 Enantioselectivity as a Function of Molecular Recognition; 1.3.1 Ionizable Molecules; 1.3.1.1 Polar Ionic Mode; 1.3.1.2 Reversed-phase Mode; 1.3.2 Neutral Molecules; 1.4 Complementary Effects; 1.5 Method Development |
1.6 Optimization Procedures1.6.1 Polar Ionic Mode; 1.6.2 Reversed-phase Mode; 1.6.2.1 pH Effects; 1.6.2.2 Organic Modifier Effects; 1.6.3 Polar Organic/Normal-phase Mode; 1.6.4 Flow-rate and Temperature Effects; 1.7 Amino Acid and Peptide Analysis; 1.8 Conclusion; Acknowledgments; References; 2 Role of Polysaccharides in Chiral Separations by Liquid Chromatography and Capillary Electrophoresis; 2.1 Introduction; 2.2 Structures of Polysaccharide Chiral Selectors; 2.2.1 Synthesis of Polysaccharide Chiral Selectors; 2.2.2 Preparation of Polysaccharide Chiral Stationary Phases |
2.2.2.1 Preparation of CSPs by Coating2.2.2.2 Preparation of CSPs by Immobilization; 2.2.2.3 Coated versus Immobilized CSPs; 2.3 Properties of Polysaccharide CSPs; 2.3.1 Enantioselectivities; 2.3.2 Spectroscopic Studies; 2.4 Applications; 2.4.1 Analytical Separations; 2.4.2 Preparative Separations; 2.5 Optimization of Chiral Separations; 2.5.1 Mobile Phase Compositions; 2.5.2 pH of the Mobile Phase; 2.5.3 Flow-rate; 2.5.4 Temperature; 2.5.5 Structures of Solutes; 2.5.6 Other Parameters; 2.6 Chiral Recognition Mechanisms; 2.7 Chiral Separation by Sub- and Supercritical Fluid Chromatography |
2.8 Chiral Separation by Capillary Electrochromatography2.9 Chiral Separation by Thin-layer Chromatography; 2.10 Chiral Separation by Capillary Electrophoresis; 2.11 Conclusion; References; 3 Analytical and Preparative Potential of Immobilized Polysaccharide-derived Chiral Stationary Phases; 3.1 Introduction; 3.1.1 Scientific Developments in Polysaccharide Immobilization with Chiral Recognition Purposes; 3.1.2 State of the Art on Immobilized Polysaccharide-derived CSPs; 3.2 Scope of Immobilized Polysaccharide-derived CSPs |
3.3 Beneficial Characteristics of Immobilized Polysaccharide-derived CSPs3.3.1 New Selectivity Profile on Immobilized CSPs; 3.3.2 Universal Miscibility of Non-standard Solvents and their Contribution to the Performance of Analytical Methods; 3.3.3 Various Sample Injection Media; 3.3.4 Inhibition or Minimization of Racemization by Mobile Phase Switch; 3.3.5 Preparative Potential of Immobilized CSPs; 3.3.6 CSP Stability; 3.4 Method Development on Immobilized Polysaccharide-derived CSPs; 3.4.1 Selection of the Mobile Phase; 3.4.1.1 Analytical Method Development |
3.4.1.2 Preparative Method Development |